Rate My Professor Wolfram Gruhn

Gruhn's laboratory focuses on the molecular mechanisms that promote human germline development, particularly epigenetic remodelling in primordial germ cells (PGCs). The research addresses key questions: what transcription factor networks drive human PGC development? How do PGCs decide which epigenetic information to erase or maintain? And can maternal environmental factors or diseases impact this process? To investigate these, the lab employs human embryonic stem cell-based models to recapitulate early germline development, integrating techniques such as RNA-seq, CUT&RUN, inducible degrons, and CRISPR genome editing. The ultimate goal is to identify risk factors contributing to infertility and abnormal germline development. Gruhn is recognized as a Principal Investigator in Biomedical Sciences and supervises PhD students through programs like the Midlands Integrative Biosciences Training Partnership (MIBTP). His contributions include high-impact publications, such as "Epigenetic resetting in the human germline entails histone modification remodelling" (Science Advances, 2023, with Walfred W. C. Tang et al.); "Specification of human germ cell fate with enhanced progression capability supported by hindgut organoids" (Cell Reports, 2023, with João Pedro Alves-Lopes et al.); "Sequential enhancer state remodelling defines human germline competence and specification" (Nature Cell Biology, 2022, with Walfred W. C. Tang et al.); "Metabolic regulation of pluripotency and germ cell fate through α-ketoglutarate" (EMBO Journal, 2019, with Julia Tischler et al.); "Targeted DamID reveals differential binding of mammalian pluripotency factors" (Development, 2018, with Seth W. Cheetham et al.); and "Gadd45g functions in male sex determination by promoting p38 signaling and Sry expression" (Developmental Cell, 2012, with Mathias S. Gierl et al.).